Daniel Lopez Laboratory

Publications

The Induction of Natural Competence Adapts Staphylococcal Metabolism to Infection

Nature Communications 13(1):1525 (2022)
Garcia J., Cordero M., Acosta I., Yepes A., Avedano J., Lisowski C., Ostereicht B., Ohlsen K., Collazo E., Föstner K., Eulalio A. and Lopez D.

DOI: 10.1038/s41467-022-29206-7.
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Substrate interaction with the EssC coupling protein of the Type VIIb secretion system

Journal of Bacteriology 202(7): e00646-19 (2020)
Mietrach N., Damián-Aparicio D., Mielich-Süß B., López D., and Geibel S.

DOI: 10.1128/JB.00646-19.
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Cell Heterogeneity in Staphylococcal Communities

Journal of molecular biology 431(23):4699-4711 (2019)
García-Betancur J. C. and Lopez D.

DOI: 10.1016/j.jmb.2019.06.011.
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Structural basis of denuded glycan recognition by SPOR domains in bacterial cell division

Nature communications 10:5567 (2019)
Alcorlo M., Dik D., De Benedetti S., Mahasenan K., Lee M., Dominguez-Gil T., Hesek D., Lastochkin E., Lopez D., Boggess W., Mobashery S., and Hermoso J.

DOI: 10.1038/s41467-019-13354-4
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Collapse of genetic division of labour and evolution of autonomy in pellicle biofilms

Nature Microbiology. 2018 Dec;3(12):1451-1460 (2018)
Dragoš A., Martin M., Falcón-Garcia C., Kricks L., Pausch P., Heimerl T., Bálint B., Maróti G., Bange G., López D., Lieleg O., Kovács A. T.

DOI: 10.1038/s41564-018-0263-y
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Structural changes of TasA in biofilm formation of Bacillus subtilis

PNAS 115 (13) 3237-3242 (2018)
Diehl A., Roske Y., Ball L., Chowdhury A., Hiller M., Molière N., Kramer R., Stöppler D., Worth C. L., Schlegel B., Leidert M., Cremer N., Erdmann N., López D., Stephanowitz H., Krause E., van Rossum E. B., Schmieder P., Heinemann U., Turgay K., Akbey U., Oschkinat H.

DOI: https://doi.org/10.1073/pnas.1718102115
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Identification of Staphylococcus aureus genes involved in the formation of structured macrocolonies

Microbiology SGM 164: 801-815 (2018)
Wermser C. H., López D.

DOI: https://dx.doi.org/10.1099/mic.0.000660
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Cell differentiation defines acute and chronic infection cell types in Staphylococcus aureus

eLIFE 6:e28023 (2017)
García-Betancur J.C., Goñi-Moreno A., Horger T., Schott M., Sharan M., Eikmeier J., Wohlmuth B., Zernecke A., Ohlsen K., Kuttler C., Lopez D.

DOI: https://doi.org/10.7554/eLife.28023.001
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Membrane Microdomain Disassembly Inhibits MRSA Antibiotic Resistance

Cell 171 (6): 1354–1367 (2017)
García-Fernández E., Koch G., Wagner R.M., Fekete A., Stengel S.T., Schneider J., Mielich-Süss B., Geibel S., Markert S.M., Stigloher C., Lopez D.

DOI: http://dx.doi.org/10.1016/j.cell.2017.10.012
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Flotillin scaffold activity contributes to type VII secretion system assembly in Staphylococcus aureus

PLoS Pathogens 13(11): e1006728 (2017)
Mielich-Süss B., Wagner R.M., Mietrach N., Hertlein T., Marincola G., Ohlsen K., Geibel S., Lopez D.

DOI: https://doi.org/10.1371/journal.ppat.1006728
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Attenuating Staphylococcus aureus Virulence by Targeting Flotillin Protein Scaffold Activity

Cell Chemical Biology 24 (7): 845-857 (2017)
Koch G., Wermser C., Acosta I.C., Kricks L., Stengel S.T., Yepes A., Lopez D.

DOI: http://dx.doi.org/10.1016/j.chembiol.2017.05.027
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Exploring Functional Membrane Microdomains in Bacteria: An overview

Current Opinion in Microbiology 36: 76-84 (2017)
Lopez D., Koch G.

10.1007/s00232-016-9923-0
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Functional Membrane Microdomains Organize Signaling Networks in Bacteria

The Journal of membrane Biology pp 1-12 (2016)
Wagner R., Kricks L., Lopez D.

10.1007/s00232-016-9923-0
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Purification of Lipid Rafts from Bacterial Membranes

Hydrocarbon and Lipid Microbiology Protocols pp 113-122 (Springer Protocols Handbook) (2016)
Wermser C., Lopez D.

DOI 10.1007/8623_2015_49
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Molecular composition of bacterial lipid rafts

Chemistry and Physics of Lipids 192: 3-11 (ORNL Workshop on Biomembranes) (2015)
Lopez D.

10.1016/j.chemphyslip.2015.08.015
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In vivo characterization of the scaffold activity of flotillin on the membrane kinase KinC of Bacillus subtilis

Microbiology 161(9):1871-87. (2015)
Schneider J, Mielich-Süss B, Böhme R and Lopez D.

DOI: 10.1099/mic.0.000137
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Individual Constituents from Essential Oils Inhibit Biofilm Mass Production by Multi-Drug Resistant Staphylococcus aureus

Molecules (2015)
Espina L, Pagán R, Lopez D, Garcia-Gonzalo D.

DOI: 10.3390/molecules200611357
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Spatio-temporal Remodeling of Functional Membrane Microdomains Organizes the Signaling Networks of a Bacterium

PLoS Genetics 11: e1005140 (2015)
Schneider J, Klein T, Mielich-Süss B, Koch G, Franke C, Kuipers OP, Kovács AT, Sauer M and Lopez D.

DOI: 10.1371/journal.pgen.1005140
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Exploring the Existence of Lipid Rafts in Bacteria

Microbiology and Molecular Biology Reviews (2015) vol. 79 (1) pp. 81-100
Bramkamp M and Lopez D.

DOI: 10.1128/MMBR.00036-14
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Evolution of resistance to a last-resort antibiotic in Staphyloccocus aureus via bacterial competition

Cell (2014) 158:1060-1071
Koch G, Yepes A, Förstner KU, Wermser C, Stengel S, Modamio S, Ohlsen K, Foster K, Lopez D.

DOI: 10.1016/j.cell.2014.06.046
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Molecular mechanisms involved in Bacillus subtilis biofilm formation

Environmental Microbiology (2014) DOI: 10.1111/1462-2920.12527
Mielich-Süss B, Lopez D.

DOI: 10.1111/1462-2920.12527
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Reconstruction of MreB expression in Staphylococcus aureus using a collection of new integrative plasmids

Applied and Environmental Microbiology (2014) 80:3868-3878
Yepes A, Koch G, Waldvogel A, Garcia-Betancur JC, Lopez D.

DOI: 10.1128/AEM.00759-14
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Structural and Functional Analysis of Bacillus subtilis YisP Reveals a Role of Its Product in Biofilm Production

Chemistry and Biology (2014) 21 (11):1557–1563
Feng X, Hu Y, Zheng Y, Zhu W, Li K, Huang CH, Ko TP, Ren F, Chan HC, Nega M, Bogue S, López D, Kolter R, Götz F, Guo RT and Oldfield E.

DOI: 10.1016/j.chembiol.2014.08.018
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Overproduction of Flotillin Influences Cell Differentiation and Shape in Bacillus subtilis

mBio (2013) 4(6): e00719-13
Mielich-Suss B, Schneider J, Lopez D.

DOI: 10.1128/mBio.00719-13
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A semi-quantitative model of Quorum-Sensing in Staphylococcus aureus approved by microarray meta analyses and tested by mutation studies

Molecular Biosystems (2013) (9): 2665-2680
Audretsch C, Lopez D, Srivastava M, Wolz C, Dandekar T.

DOI: 10.1039/c3mb70117d
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The Biofilm Formation Defect of a Bacillus subtilis Flotillin-defective Mutant Involves the Protease FtsH

Molecular Microbiology (2012) 86 (2): 457-471
Yepes A, Schneider J, Mielich B, Koch G, García-Betancur. JC, Ramamurthi KS, Vlamakis H, Lopez D.

DOI: 10.1111/j.1365-2958.2012.08205.x
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Single-cell analysis of Bacillus subtilis biofilms using fluorescence microscopy and flow cytometry

Journal of Visualized Experiments (2012) (60): 1-8
García-Betancur JC, Yepes A, Schneider J, Lopez D.

DOI: 10.3791/3796
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Streptomycin-induced expression in Bacillus subtilis of YtnP, a lactonase-homologous protein that inhibits development and streptomycin production in Streptomyces griseus

Applied and Environmental Microbiology (2012) (78(2): 599-603
Schneider J, Yepes A, Garcia-Betancur JC, Westedt I, Mielich B, Lopez D.

DOI: 10.1128/AEM.06992-11
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Microbes at their best: first Mol Micro Meeting Würzburg

Molecular Microbiology (2011) 82(4): 797-806
Böhm A, Papenfort K, López D and Vogel J.

DOI: 10.1111/j.1365-2958.2011.07852.x
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Antibiotics as Signal Molecules

Chemical Reviews (2011) 111(9): 5492-5505
Romero D, Traxler MF, Lopez D and Kolter R.

DOI: 10.1021/cr2000509
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Functional microdomains in bacterial membranes

Genes and Development (2010) 24(17): 1893-1902
Lopez D, Kolter R.

DOI: 10.1101/gad.1945010
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Cold Spring Harbor Perspectives in Biology

Biofilms (2010) 2(7): a000398
Lopez D, Vlamakis H, Kolter R.

DOI: 10.1371/journal.pgen.1005140

Extracellular signals that define distinct and coexisting cell fates in Bacillus subtilis

FEMS Microbiology Reviews (2010) 34(2): 134-149
Lopez D, Kolter R.

DOI: 10.1111/j.1574-6976.2009.00199.x
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Potassium sensing histidine kinase in Bacillus subtilis

Methods Enzymology (2010) 471: 229-251
Lopez D, Gontang EA, Kolter R.

DOI: 10.1016/S0076-6879(10)71013-2
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Paracrine signaling in a bacterium

Genes and Development (2009) 23(14): 1631-1638
Lopez D, Vlamakis H, Losick R, Kolter R.

DOI: 10.1101/gad.1813709
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Cannibalism enhances biofilm development in Bacillus subtilis

Molecular Microbiology (2009) 74(3): 609-618
Lopez D, Vlamakis H, Losick R, Kolter R.

DOI: 10.1111/j.1365-2958.2009.06882.x
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Generation of multiple cell types in Bacillus subtilis

FEMS Microbiology Reviews (2009) 33(1): 152-163
Lopez D, Vlamakis H, Kolter R.

DOI: 10.1111/j.1574-6976.2008.00148.x
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Structurally diverse natural products that cause potassium leakage trigger multicellularity in B. subtilis

Proc Natl Acad Sci USA (2009) 106(1): 280-285
Lopez D, Fischbach MA, Chu F, Losick R, Kolter R.

DOI: 10.1073/pnas.0810940106
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Involvement of a novel copper chaperone in tyrosinse activity and melanin synthesis in Marinomonas mediterranea

Microbiology (2007) 153:2241-2249
Lopez D, Solano F, Sanchez-Amat A.

DOI: 10.1099/mic.0.2007/006833-0
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Identification of an operon involved in tyosinase activity and melanin synthesis in Marinonomas mediterranea

Gene (2004) 342:179-187
Lopez D, Solano F and Sánchez-Amat A.

DOI: 10.1371/journal.pgen.1005140

Marinomonas mediterraea is a lysogenic bacterium that synthesizes R-bodies

Microbiology (2003) 149:2679-2686
Hernández-R. D, Lucas P, Lopez D, Solano F, Sánchez-Amat A.

DOI: 10.1099/mic.0.26524-0
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Cloning and molecular characterization of a SDS-activated tyrosinase from M. mediterranea

Pigment Cell and Melanoma Research (2002) 15:104-111
Lopez D, Sánchez-Amat A and Solano F.

DOI: 10.1034/j.1600-0749.2002.1o068.x
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Dimethoxyphenol oxidase activity of different microbial blue multicopper proteins

FEMS Microbiology Letters (2001) 204:175-181
Solano F, Lucas P, Lopez D, Fernandez E and Sánchez-Amat A.

DOI: 10.1111/j.1574-6968.2001.tb10882.x
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Patents

Inhibitors of biofilm formation.

Patent Number: WO/2011/031964
Publication Date: 17.03.2011
Designated States: International patent.
López D, Kolter R and Hatton B.

Inhibitors of biofilm formation Issuing year: 2012.

Patent Number: USSN 61/241,342
International patent. PCT/US10/48422.
Publication Date: 2012
Lopez D, Kolter R and Hatton B

New use of statins in overcoming resistance to β-lactam antibiotics in bacterial species synthetizing isoprenoids using the mevalonate synthetic pathway Issuing year.

Patent Number: WO2019086633
Publication Date: 2017
Lopez D and Koch G

Publications

The Induction of Natural Competence Adapts Staphylococcal Metabolism to Infection

Substrate interaction with the EssC coupling protein of the Type VIIb secretion system

Cell Heterogeneity in Staphylococcal Communities

Structural basis of denuded glycan recognition by SPOR domains in bacterial cell division

Collapse of genetic division of labour and evolution of autonomy in pellicle biofilms

Structural changes of TasA in biofilm formation of Bacillus subtilis

Identification of Staphylococcus aureus genes involved in the formation of structured macrocolonies

Cell differentiation defines acute and chronic infection cell types in Staphylococcus aureus

Membrane Microdomain Disassembly Inhibits MRSA Antibiotic Resistance

Flotillin scaffold activity contributes to type VII secretion system assembly in Staphylococcus aureus

Attenuating Staphylococcus aureus Virulence by Targeting Flotillin Protein Scaffold Activity

Exploring Functional Membrane Microdomains in Bacteria: An overview

Functional Membrane Microdomains Organize Signaling Networks in Bacteria

Purification of Lipid Rafts from Bacterial Membranes

Molecular composition of bacterial lipid rafts

In vivo characterization of the scaffold activity of flotillin on the membrane kinase KinC of Bacillus subtilis

Individual Constituents from Essential Oils Inhibit Biofilm Mass Production by Multi-Drug Resistant Staphylococcus aureus

Spatio-temporal Remodeling of Functional Membrane Microdomains Organizes the Signaling Networks of a Bacterium

Exploring the Existence of Lipid Rafts in Bacteria

Evolution of resistance to a last-resort antibiotic in Staphyloccocus aureus via bacterial competition

Molecular mechanisms involved in Bacillus subtilis biofilm formation

Reconstruction of MreB expression in Staphylococcus aureus using a collection of new integrative plasmids

Structural and Functional Analysis of Bacillus subtilis YisP Reveals a Role of Its Product in Biofilm Production

Overproduction of Flotillin Influences Cell Differentiation and Shape in Bacillus subtilis

A semi-quantitative model of Quorum-Sensing in Staphylococcus aureus approved by microarray meta analyses and tested by mutation studies

The Biofilm Formation Defect of a Bacillus subtilis Flotillin-defective Mutant Involves the Protease FtsH

Single-cell analysis of Bacillus subtilis biofilms using fluorescence microscopy and flow cytometry

Streptomycin-induced expression in Bacillus subtilis of YtnP, a lactonase-homologous protein that inhibits development and streptomycin production in Streptomyces griseus

Microbes at their best: first Mol Micro Meeting Würzburg

Antibiotics as Signal Molecules

Functional microdomains in bacterial membranes

Cold Spring Harbor Perspectives in Biology

Extracellular signals that define distinct and coexisting cell fates in Bacillus subtilis

Potassium sensing histidine kinase in Bacillus subtilis

Paracrine signaling in a bacterium

Cannibalism enhances biofilm development in Bacillus subtilis

Generation of multiple cell types in Bacillus subtilis

Structurally diverse natural products that cause potassium leakage trigger multicellularity in B. subtilis

Involvement of a novel copper chaperone in tyrosinse activity and melanin synthesis in Marinomonas mediterranea

Identification of an operon involved in tyosinase activity and melanin synthesis in Marinonomas mediterranea

Marinomonas mediterraea is a lysogenic bacterium that synthesizes R-bodies

Cloning and molecular characterization of a SDS-activated tyrosinase from M. mediterranea

Dimethoxyphenol oxidase activity of different microbial blue multicopper proteins

Patents

Inhibitors of biofilm formation.

Inhibitors of biofilm formation Issuing year: 2012.

New use of statins in overcoming resistance to β-lactam antibiotics in bacterial species synthetizing isoprenoids using the mevalonate synthetic pathway Issuing year.

**Identification of Staphylococcus aureus genes involved in the formation of structured macrocolonies**

**Evolution of resistance to a last-resort antibiotic in Staphyloccocus aureus via bacterial competition**

**Reconstruction of MreB expression in Staphylococcus aureus using a collection of new integrative plasmids**

**A semi-quantitative model of Quorum-Sensing in Staphylococcus aureus approved by microarray meta analyses and tested by mutation studies**

**The Biofilm Formation Defect of a Bacillus subtilis Flotillin-defective Mutant Involves the Protease FtsH**

**Single-cell analysis of Bacillus subtilis biofilms using fluorescence microscopy and flow cytometry**